Normalize intensities across samples using standard Quantile Normalization

This is the standard approach for Quantile Normalization. Other sub-flavors are also available:

See References for more information.

Usage

normalize_quantile_all(data)

Arguments

data: A tidy tibble created by read_featuretable.

Value

A tibble with intensities normalized across samples.

References

Y. Zhao, L. Wong, W. W. B. Goh, Sci Rep 2020, 10, 15534, DOI 10.1038/s41598-020-72664-6.

Examples

toy_metaboscape %>%
  normalize_quantile_all()
#> # A tibble: 110 × 8
#>      UID Feature                Sample  Intensity    RT `m/z` Name       Formula
#>    <int> <chr>                  <chr>       <dbl> <dbl> <dbl> <chr>      <chr>  
#>  1     1 161.10519 Da 26.98 s   Sample1      4.31  0.45  162. NA         C7H15N…
#>  2     2 276.13647 Da 27.28 s   Sample1      3.14  0.45  277. Octyl hyd… C16H22…
#>  3     3 304.24023 Da 32.86 s   Sample1      8.12  0.55  305. Arachidon… C20H32…
#>  4     4 417.23236 Da 60.08 s   Sample1      6.04  1     418. NA         NA     
#>  5     5 104.10753 Da 170.31 s  Sample1      6.04  2.84  105. NA         C5H14NO
#>  6     6 105.04259 Da 199.80 s  Sample1      6.04  3.33  106. NA         C3H8NO3
#>  7     7 237.09204 Da 313.24 s  Sample1      9     5.22  238. Ketamine   C13H16…
#>  8     8 745.09111 Da 382.23 s  Sample1      3.14  6.37  746. NADPH      C21H30…
#>  9     9 427.02942 Da 424.84 s  Sample1      4.31  7.08  428. ADP        C10H15…
#> 10    10 1284.34904 Da 498.94 s Sample1    NaN     8.32 1285. NA         NA     
#> # ℹ 100 more rows